Extraction and detection of mRNA from horsehair.

NOTE Internal Medicine Extraction and Detection of mRNA from Horsehair

After RNA extraction from horsehair shafts and roots, the mRNAs of β-actin, muscle-type phosphofructokinase, and transforming growth factor-β1 were detected by reverse transcription polymerase chain reaction assay. Low amounts of RNA were present in the horsehair. These specific mRNA transcripts were readily detected when more than three hair roots were used. However, detection of the mRNA tran...

RNA extraction and leptin receptor mRNA detection in bull ejaculated spermatozoa

Development of a Multiplex RT-PCR Assay for Detection of the Causal Agents of Citrus Tristeza and Cachexia Diseases with Coamplification of Plant mRNA as an Internal Control

Background and Aims: Plant certification programs need reliable, fast, cheap and sensitive methods for detection of systemic pathogens with special interest in virus and viroid detection. Reverse transcriptase-polymerase chain reaction (RT-PCR) has been documented as an alternative assay for certification of plant propagating materials. The main object of the present study was the optimization ...

Polyaniline/Graphene Nanocomposite as a Promising Sorbent for Dispersive Solid Phase Extraction of Avermectins from Citrus Fruit Juice

A solid phase extraction sorbent based on polyaniline/graphene nanocomposite is presented. The structure and morphology of synthesized nanocomposite were investigated by Fourier transform infrared spectroscopy, field emission scanning electron microscopy, X-ray diffraction and thermal gravimetric analysis. The dispersive solid phase extraction was employed to the isolation and preconcentration ...

Extraction of Sulfathiazole from Urine Using Biosynthesized Magnetic Nanoparticles

The application of Pd/Fe3O4 nanoparticles (NPs) for the adsorption of sulfathiazole from urine samples prior to high performance liquid chromatography-ultraviolet detection was studied. Pd/Fe3O4 NPs were synthesized using plant extract. Possible impact parameters in the extraction process such as the magnetic adsorbents amount, extraction time, sample pH and desorption conditions were investiga...